Prelimbic Ensembles Mediate copyright Seeking After Behavioral Acquisition and Once Rats Are Well-Trained

Prelimbic Ensembles Mediate copyright Seeking After Behavioral Acquisition and Once Rats Are Well-Trained

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Substance use disorder (SUD) is a chronic relapsing condition characterized by continued use of drugs despite negative consequences.SUD is thought to involve disordered learning and memory wherein drug-paired cues gain increased salience, and ultimately drive craving and relapse.These types of associations are thought to be encoded within sparsely distributed sets of neurons, called neuronal ensembles, that drive encoded behaviors through synchronous activity of the participant neurons.We have Wooden Car previously found that Fos-expressing neuronal ensembles within the prefrontal cortex are required for well-trained copyright seeking.

However, less is known about how quickly cortical neuronal ensembles form during the initiation of copyright seeking behavior.Here, we seek to further elucidate the role of Fos-expressing neuronal ensembles within the prelimbic cortex (PL) after the initial acquisition of copyright self-administration (SA), or, after 10 days of additional SA training (well-trained).We trained Fos-LacZ transgenic rats to lever press for copyright under an FR1 schedule of reinforcement.Once rats met acquisition criteria for copyright self-administration, we ablated Fos-expressing neuronal ensembles in the PL using the Daun02 inactivation method, either 1 or 10 days after the rats met the acquisition criteria.

Targeted ablation here of Fos-expressing neuronal ensembles in the PL attenuated active lever pressing both 1 day and 10 days after rats acquired copyright self-administration.Together, this suggests that Fos-expressing neuronal ensembles rapidly form in the PL and continue to mediate maintained copyright seeking behavior.